Biochemistry of neurosensory processes/ signal transduction

NCS proteins

Neuronal calcium sensor (NCS) proteins constitute a family of proteins, which can be further subdivided into five subfamilies. A common feature of NCS proteins is the presence of 4 EF-hand motifs (calcium binding region), which consist of a loop region (12 amino acids) that is flanked by two a-helices (see figure). Almost all NCS proteins harbour at the N-terminus a consensus site for cotranslational myristoylation. Prototype of the NCS protein family is recoverin that is mainly expressed in the vertebrate retina. Another subfamily contains the GCAPs (guanylate cyclase-activating proteins) that regulate the activities of membrane bound guanylate cyclases in the vertebrate retina.

 

 

Selected publications

Fries, R., Reddy, P., P., Mikhaylova, M., Haverkamp, S., Wei, T., Müller, M., Kreutz. M. R. and Koch, K.-W. (2010) Dynamic cellular translocation of caldendrin is facilitated by the Ca2+ -myristoyl switch of recoverin. J Neurochem. 113, 1150-1162

Rätscho, N., Scholten, A. and Koch, K.-W. (2009) Expression profiles of three novel sensory guanylate cyclases and guanylate cyclase-activating proteins in the zebrafish retina.BBA- Molecular Cell Research1793, 1110-1114

Rätscho, N., Scholten A., Koch,K.-W. (2010) Diversity of sensory guanylate cyclases in teleost fishes. Mol Cell Biochem. 334, 207-214

Komolov, K. E., Senin, I. I., Kovaleva, N. A., Christoph, M.P., Churumova, V.A., Grogoriev, I. I., Akhtar, M., Philippov, P. P. and Koch, K.-W. (2009) Mechanism of rhodopsin kinase regulation by recoverin. J Neurochem. 110, 72-79

Zernii, E., Komolov, K., Permyakov S., Kolpakova, T., Dell´Orco, D., Poetzsch, A., Knyazeva, E., Grigoriev, I., Permyakov, E., Senin, I., Philippov, P. and Karl-Wilhelm Koch (2011) Involvement of recoverin C-terminal segment in recognition of the target enzyme rhodopsin kinase. Biochem. J., 435,441-45